Many reports have claimed that freeze-dried sperm which is not motile but has an intact DNA was able to fertilize oocytes, even produced offspring in mouse. The sperm resulted from this technique is in drying state, therefore, it might be stored in room temperature or in refrigerator. The freeze-drying method is an alternative method in sperm preservation which ignored the motility of sperm. Due to the benefit of ICSI over the conventional in vitro fertilization (IVF) the spermatozoon could be mechanically driven to pass through the zona pellucida and entering the cytoplasm of oocytes prior to fertilization. The application of intracytoplasmic sperm injection (ICSI) for which sperm motility is unnecessary had supported the effort to create simplified method such as freeze-drying for sperm preservation. Sehingga dari sini dapat terlihat bahwa pertumbuhan dan perkembangan adalah sesuatu yang berbeda tetapi saling berkesinambungan atau berhubungan. This technology was then applied to the preservation of embryo, oocyte, ovary and testis. Yang dimaksud perubahan fisik pada perkembangan manusia ialah mengacu pada optimaliasasi fungsi-fungsi organ jasmaniah manusia, bukan pada pertumbuhan jasmaniah itu sendiri. Tidak tampak bentuk-bentuk baru pada saat ini. Since the discovery of cryopreservation method for bull semen, cryopreservation become an alternative method for maintaining gamet resources of certain animal which is threatened or near extinction. Berikut pertumbuhan dan perkembangan pada hewan dan manusia selama fase janin : Terjadi perubahan pada bagian-bagian tubuh yang telah terbentuk, baik dalam bentuk maupun perubahan aktual, serta terjadi perubahan dalam fungsi.
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